High Performance Liquid Chromatography

High Performance Liquid Chromatography (HPLC) and Ultra High Performance Liquid Chromatography (UHPLC) use liquid mobile phases to transport the sample components through a column packed with a solid material; the stationary phase. The "mode" of chromatography is usually defined by a combination of a certain stationary phase type with a certain mobile phase type. In order to generate a chromatogram that is suitable for qualitative or quantitative purposes, bonded phase chemistry, column dimensions, and particle and pore sizes are parameters that need to be optimized.